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Data de entrada: 18 de mai. de 2022

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Extensions.Interchange of P2X7 receptor mediated calcium signalling with Na+ and K+ fluxes in an immortalized cell line derived from CD14+



 

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Need help with Web Builder extensions? If you're looking for a particular extension in Web Builder, check out our catalog of WYSIWYG Web Builder Extensions.Interchange of P2X7 receptor mediated calcium signalling with Na+ and K+ fluxes in an immortalized cell line derived from CD14+ peripheral blood monocytes. P2X7 receptor activation leads to a rapid rise in intracellular calcium ([Ca(2+)](i)) in neutrophils and monocytes, but the functional consequences of this response are still unclear. In the present work, we characterized the [Ca(2+)](i) dynamics triggered by P2X7 receptor activation in an immortalized cell line derived from CD14(+) peripheral blood monocytes. We report that a short (1 min) P2X7 receptor stimulation by ATP triggers a transient rise in [Ca(2+)](i) that persists for 30 min. The Na(+)-Ca(2+) exchanger inhibitor NKA and the intracellular calcium chelator BAPTA inhibited the sustained phase of the [Ca(2+)](i) increase, suggesting that this sustained phase is due to the rapid Na(+) influx associated with the activation of P2X7 receptors. The activation of P2X7 receptors led to a sustained increase in intracellular Na(+) and a decrease in extracellular K(+) that was concentration-dependent. Preincubation of the cells with the P2X7 receptor blocker BBG inhibited the ATP-induced increase in Na(+) and K(+) fluxes. We also demonstrate that ATP-induced [Ca(2+)](i) increases occur in the absence of extracellular Ca(2+) suggesting that the P2X7 receptor induces an internal Ca(2+) store release. The present study indicates that P2X7 receptor activation triggers a Ca(2+) mobilization associated with Na(+) and K(+) efflux in the plasma membrane.Three speakers who refused to apologize for their support of the boycott have been barred from the university. In October, the three Kent State University (KSU) professors — Dave Kunzelman, Steve Graham, and Nathan Wissner — made headlines after their university refused to issue them student identification cards because of their boycott activism. In a public statement, the professors explained their reason for refusing to issue student IDs in a letter addressed to KSU President Lester Lefton, published




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